The 53-year-old myotherapist and personal trainer has been remembered by her son Matthew who shared a tribute on Facebook.

"To the most loving, most caring person I've ever known can't thank you enough for everything you ever did for me and all the support you gave me, words can't begin to describe how much I will miss you, wish I could give you one last hug," he said.

"Love you infinity Mum."

Meanwhile, the business made a statement on the incident.

In an image posted to Facebook, Soul Barn, a wellness store on the town's main street, said none of its regular therapists, staff or facilitators were at the business at the time of the death.

"There are no words to express the deep sorrow and shock we are feeling here at Soul following the tragic incident that occurred on April 13," the post said.

"Soul Barn hires out workshop space to external businesses and facilitators. The event which took place on April 13th was a private event, and those facilitating the event do not work for or represent Soul Barn in any way. None of our regular therapists, staff or facilitators were present at any point during this event.

"We share the shock and devastation of everyone involved, and our hearts are with those families affected. Soul Barn is closed while police complete their investigation. We have no further information to share, and we ask that you respect the privacy of everyone in our small community. Thank you."

Detectives from the Moorabool Crime Investigation Unit are investigating the incident which happened on Fraser Street in Clunes, north of Ballarat, about midday on April 13.

"It is believed a woman was at a retreat on Fraser Street when she became ill after ingesting a drink," a Victoria Police spokesperson said.

"The 53-year-old Ringwood North woman died at the scene... two other people were taken to hospital for observation.

"Investigations remain ongoing and police will prepare a report for the Coroner."

It is not known what sort of drink the woman had.

Anyone who witnessed the incident or has information is urged to phone Crime Stoppers on 1800 333 000.

A confidential report can also be made online at crimestoppersvic.com.au